Our lab is creating a series of Gal4-UAS and LexA-LOP based bipartite transgenic lines specifically designed to facilitate studies of the nervous system. The use of multiple bipartite expression systems will allow apposing neuronal subsets to be labeled and/or manipulated independently.
Neural Restrictive Silence Element (NRSE) Lines: Incorporating NRSE sites into Gal4 (or LexA) driver transgenes serves to delimit transgene expression to the nervous system (Xie et al., BMC Biology, 2012), eliminating problematic 'background' expression in skeletal muscle and heart commonly observed in Gal4-based enhancer trap lines. By restricting expression of bipartite drivers to neural tissues, NRSE-delimited lines provide a means to more discretely modulate targeted neural cell subtypes.
This website is intended to assist members of the research community in determining whether a specific transgenic line could enhance their research. High-resolution expression pattern images and insertion site data will be provided as it becomes available. This work was supported by a grant award from the NIH (R21 MH083614).
Yellow = membrane tagged YFP (denotes the expression of the driver transgene, Red = Texas Red Bodipy (provides contextual information), Cyan = Tg(pax6-DF4:gap43-CFP)q01, a transgenic line providing contextual information via near ubiquitous expression of membrane tagged CFP. Note that membrane tagged reporters produce strong labeling in synaptic neuropils, cell bodies appear as hollow spheres.
Phenotypes:
Neural = Restricted to Neural Tissue (in some cases not until larval stages, see Xie at al., 2012, BMC Biology)
Mixed = Expression in Neural and non-Neural Tissues
Non-Neural = Expression in Non-Neural Tissues Only
Grouped images are arrayed Dorsal, Lateral, and Ventral (top to bottom)
Line
Trapped enhancer / Phenotype
gmc601
tbd / Neural
gmc602
tbd / Mixed
gmc603
tbd / Neural
gmc604
tbd / Neural
gmc605
tbd / Neural
gmc606
tbd / Mixed
gmc607
tbd / Neural
gmc608
tbd / Neural
gmc609
tbd / Mixed
gmc610
tbd / Neural
gmc611
tbd / tbd
gmc612
tbd / tbd
gmc613
tbd / Mixed
gmc614
tbd / Mixed
gmc615
tbd / Mixed
gmc616
tbd / Mixed
gmc617
tbd / Neural
gmc618
tbd / Mixed
gmc619
tbd / Mixed
gmc620
tbd / Neural
gmc621
tbd / Neural
gmc622
tbd / Neural
gmc623
tbd / Neural
gmc624
tbd / Neural
gmc631
tbd / Neural
gmc632
tbd / Mixed
gmc641
tbd / Neural
gmc642
tbd / Neural
gmc675
tbd / Mixed
gmc676
tbd / Mixed
gmc677
tbd / Mixed
gmc678
tbd / Neural
gmc679
tbd / Mixed
gmc680
tbd / Mixed
gmc681
tbd / Mixed
gmc682
tbd / Mixed
gmc683
tbd / Mixed
gmc684
tbd / Non-neural
gmc685
tbd / Mixed
gmc686
tbd / Mixed
gmc687
tbd / Non-neural
gmc688
tbd / Mixed
gmc689
tbd / Mixed
gmc690
tbd / Mixed
gmc691
tbd / Mixed
gmc692
tbd / Mixed
gmc693
tbd / Mixed
gmc694
tbd / Mixed
gmc695
tbd / Mixed
gmc696
tbd / Mixed
gmc697
tbd / Mixed
gmc698
tbd / Mixed
gmc699
tbd / tbd
gmc725
tbd / Neural
gmc726
tbd / Neural
gmc727
tbd / Mixed
gmc728
tbd / Mixed
gmc729
tbd / Neural
gmc730
tbd / Neural
gmc731
tbd / Mixed
gmc732
tbd / Neural
gmc733
tbd / Neural
gmc734
tbd / Mixed
gmc735
tbd / Mixed
gmc736
tbd / Neural
gmc737
tbd / Mixed
gmc738
tbd / Mixed
gmc739
tbd / Neural
gmc740
tbd / Neural
gmc741
tbd / Mixed
gmc742
tbd / Neural
gmc743
tbd / Neural
gmc744
tbd / Neural
gmc745
tbd / Neural
gmc746
tbd / Non-neural
gmc747
tbd / Mixed
gmc748
tbd / Mixed
gmc749
tbd / Mixed
gmc750
tbd / Neural
gmc751
tbd / tbd
gmc752
tbd / tbd
gmc753
tbd / tbd
gmc754
tbd / tbd
gmc755
tbd / tbd
gmc756
tbd / tbd
gmc757
tbd / tbd
gmc758
tbd / tbd
gmc759
tbd / tbd
gmc774
tbd / tbd
gmc800
tbd / tbd
gmc801
tbd / tbd
gmc802
tbd / tbd
gmc803
tbd / tbd
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