Mumm Lab Zebrafish Enhancer Trap Lines
Our lab is creating a series of Gal4-UAS and LexA-LOP based bipartite transgenic lines specifically designed to facilitate studies of the nervous system. The use of multiple bipartite expression systems will allow apposing neuronal subsets to be labeled and/or manipulated independently.
Neural Restrictive Silence Element (NRSE) Lines: Incorporating NRSE sites into Gal4 (or LexA) driver transgenes serves to delimit transgene expression to the nervous system (Xie et al., BMC Biology, 2012), eliminating problematic 'background' expression in skeletal muscle and heart commonly observed in Gal4-based enhancer trap lines. By restricting expression of bipartite drivers to neural tissues, NRSE-delimited lines provide a means to more discretely modulate targeted neural cell subtypes.
This website is intended to assist members of the research community in determining whether a specific transgenic line could enhance their research. High-resolution expression pattern images and insertion site data will be provided as it becomes available. This work was supported by a grant award from the NIH (R21 MH083614).
Yellow = membrane tagged YFP (denotes the expression of the driver transgene, Red = Texas Red Bodipy (provides contextual information), Cyan = Tg(pax6-DF4:gap43-CFP)q01, a transgenic line providing contextual information via near ubiquitous expression of membrane tagged CFP. Note that membrane tagged reporters produce strong labeling in synaptic neuropils, cell bodies appear as hollow spheres.
Neural = Restricted to Neural Tissue (in some cases not until larval stages, see Xie at al., 2012, BMC Biology)
Mixed = Expression in Neural and non-Neural Tissues
Non-Neural = Expression in Non-Neural Tissues Only
Grouped images are arrayed Dorsal, Lateral, and Ventral (top to bottom)
Website adapted by Jeff Mumm from a design by Olga Lositsky & Martin Distel
� 2012 Georgia Health Sciences University