Flow Cytometry Header depics the CRC building 

Flow Cytometry Lab | News | Equipment | Flourophores | Training | Rates | FAQs | Publication Requirements | Protocols & Useful Links

Protocols & Useful Links

 Signifies key resources

Current Protocols in Cytometry Signifies key resources

 

is available - both electronic and hard copies! Contact William King for more information.

Current Protocols in Cytometry is a "best practices" collection that distills and organizes the absolute latest techniques from the top cytometry labs and specialists worldwide. It is the most complete set of peer-reviewed protocols for flow and image cytometry available. Updated every three months in all formats, Current Protocols in Cytometry is constantly evolving to keep pace with the very latest discoveries and developments. On average, 52% of content is new and 48% has been revised every year since initial publication.

Current Protocols in Immunology Signifies key resources

 

is available through the GHSU Cancer Center Investigator Yukai He, MD, PhD.

Current Protocols in Immunology is also a "best-practices" collection that provides comprehensive coverage of immunological methods from classic to the most cutting edge: the most sophisticated protocols in immunology today. Updated every three months in all formats, Current Protocols in Immunology is constantly evolving to keep pace with the very latest discoveries and developments. Since the initial publication of the work, 770 pages of content is new (43%) and revised (57%) on average every year! Current Protocols in Immunology covers the latest in antibody detection and preparation, molecular immunology, innate immunity, tumor immunology, engineering immune molecules and receptors, and more; as well as animal models for analyses; offers valuable reference information in five extensive appendices, including commonly used reagents, equipment and techniques; and a full appendix devoted to the CD system of leukocyte surface molecules.

Antibody Cross-Reactivity Resources:
Apoptosis:
Cell Cycle Basics

- from University College London

 

Cell Cycle protocols and analysis

- from University of Texas MD Anderson Cancer Center

 

CellQuest Pro User's Guide

- from Becton Dickinson

 

Compensation

- from Mario Roederer, PhD

 

Conjugation of monoclonal antibodies

- also by Mario Roederer, PhD

 

Cytometry Techniques and Protocols

(Purdue)

 

Diva Quick Start Guide

- from Becton Dickinson

 

DNA Analysis Protocols

 

Flow Cytometry on the Web

- from Salk Institute

Flow cytometry webinars & symposiums

-by Beckman Coulter


FlowJo analysis software:

 

Fluorescence Tutorials

- by Invitrogen

 

Fluorescence Spectrum Viewers: Signifies key resources
LSR II User's Guide -from BD (hosted at Stanford)

 

Fluorescent Proteins:
G-force calculator for your centrifuge

- it's the g-force that's important, not the RPMs!!

 

In Living Color

- Protocols in Flow Cytometry and Cell Sorting by R. Diamond and S. DeMaggio. The lab manager's personal copy of the book is available for review to those that inquire.

 

Intracellular cytokine protocols

- by Joost Schuitemaker

 

ModFit LT 3.3 software:

 

Purdue Cytometry ListServ:

A valuable forum for the discussion of many aspects of flow cytometry is the Purdue Cytometry Electronic Mailing List, which has an international following. Persons may search the discussion archive at http://www.cyto.purdue.edu/hmarchiv/indec.htm. To subscribe to the discussion, fill out the form at https://lists.purdue.edu/mailman/listinfo/cytometry. To post an email to the group, send an email to cytometry@flowcyt.cyto.purdue.edu.

 

Sorting Buffers

as posted at Scripps.

 

Technical Center for those using secondary and tertiary antibodies

by Jackson ImmunoResearch Laboratories, Inc.

 

Techniques for Immune Function Analysis 1st Edition

- a 248 page handbook by Becton Dickinson

 

Tissue Dissociation Guide

by Worthington Biochemical Corporation

 

Titering Antibodies

- by Carlton and Sigrid Stewart

 

VENDOR WEB SITES:

 

William King is soley responsible for the content of this site. Comments, concerns and questions regarding it should

be addressed to him.

 

Revised: 5/16/12